Coding

Part:BBa_K1111013

Designed by: Sandra Elisabeth Chaudron, Caroline Desmurget and Mareike Apelt   Group: iGEM13_EPF_Lausanne   (2013-09-19)

Ice Nucleation Protein fused to Streptavidin Dead

Introduction

It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein found in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.

Figure 1: outline of the expected outcome of the plasmid

Usage and Biology

This part was designed to express streptavidin on the outer membrane of E.coli.
It serves as a negative control as the dead Streptavidin is though to not bind to biotin or with really decreased affinity.

Datasheet

For further Characterization, refer to the datasheet below.

CharacterizationSheet Page1
CharacterizationSheet Page2























File:Team-EPF-Lausanne Sheet.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1727
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1727
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 1691
    Illegal AgeI site found at 1742
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None